详细介绍
品牌 | 其他品牌 | 规格 | Ag0032 |
---|---|---|---|
供货周期 | 一周 | 应用领域 | 医疗卫生,生物产业 |
Recombinant Human 4Ig-B7-H3 Protein,His-tag
重组人B7-H3蛋白,His-tag
Recombinant Human CD276 Protein,His-tag
重组人CD276蛋白,His-tag
PRODUCT DETAILS | ||||
Alternative Names | CD276, B7-H3, B7H3 | |||
Expression System | HEK 293 cells | |||
Purity | ≥95% SDS-PAGE | |||
Protein Length | Protein Leu29-Ala534, with a C-terminal 6-His tag. Database link: uniprot.org/uniprot/Q5ZPR3 | |||
Accession# | NP_079516 | |||
Predicted Molecular Mass | 55 kDa | |||
SDS-PAGE | 70-90 kDa | |||
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with trehalose | |||
Reconstitution | Centrifuge vial prior to opening vial. Add 0.5 mL sterile water or 0.01M PBS (pH7.2) at 0.4 mg/mL Gently pipet solution down the sides of the vial. DO NOT VORTEX. | |||
Please note: For research use only. Not for diagnostic or therapeutic use. | ||||
SHIPPING & STROAGE | ||||
Shipping | Lyophilized product is shipped at ambient temperature. | |||
Storage | Upon receipt, store immediately at -20 to -70°C:12 months from date of receipt. After reconstitution under sterile conditions: 2 to 8°C or 1 month or -20 to -70°C for 6 months. Avoid freeze-thaw cycles. | |||
APPLICATIONS![]() | ||||
Item | Description | Data Examples | ||
SDS PAGE | 10 µg recombinant human 4Ig-B7-H3(Cat.#: 0721141), his tag protein, reducing condition | ![]() |
074A031 Recombinant human B7H3 His Tag
074A032 Recombinant human B7H3 His Tag
074A033 Recombinant human B7H3 human Fc
074A034 Recombinant human B7H3 human Fc
074A035 Recombinant human B7H3 mouse Fc
074A036 Recombinant human B7H3 mouse Fc
北京汇智泰康医药技术有限公司致力于为药物、医疗器械、化妆品、新化学物质、农药、消毒产品等生产研发机构提供相关产品与服务。公司取得的资质包括 CNAS,GLP,CMA,AAALAC,化学品毒性鉴定机构等。
原核表达系统:
将克隆化基因插入合适载体后导入原核生物用于表达大量蛋白质的方法一般称为原核表达。主要包括:大肠杆菌表达系统、枯草芽孢杆菌表达系统、链霉菌表达系统等,其中应用广泛的是大肠杆菌表达系统。
酵母表达 :
酵母表达系统是研究真核蛋白质表达和分析的有力工具,拥有转录后加工修饰功能,适合于稳定表达有功能的外源蛋白质。与昆虫表达系统和哺乳动物表达系统相比,酵母表达系统操作简便,成本低廉,可大规模进行发酵。
哺乳动物细胞表达:
哺乳动物细胞表达系统的优势在于能够指导蛋白质的正确折叠,提供复杂的N型糖基化和准确的O型糖基化等多种翻译后加工功能,因而表达产物在分子结构、理化特性和生物学功能方面接近于天然的高等生物蛋白质分子。
目前已有许多重要的蛋白及糖蛋白利用哺乳动物细胞表达系统大量制备、生产。如:人组织型血纤蛋白酶原激活因子、凝血因子Ⅷ、干扰素、乙肝表面抗原、红血球生成激素、人生长激素等。
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